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rna sequencing rna seq procedure  (Macrogen)
86
Macrogen rna sequencing rna seq procedure
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Rna Sequencing Rna Seq Procedure, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pmc13259754-90-0-9?v=Macrogen
Average 86 stars, based on 1 article reviews
rna sequencing rna seq procedure - by Bioz Stars, 2026-07
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rna sequencing procedures  (Sangon Biotech)
86
Sangon Biotech rna sequencing procedures
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Rna Sequencing Procedures, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pm41844371-65-1-7?v=Sangon+Biotech
Average 86 stars, based on 1 article reviews
rna sequencing procedures - by Bioz Stars, 2026-07
86/100 stars
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rna sequencing procedures  (Novogene)
86
Novogene rna sequencing procedures
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Rna Sequencing Procedures, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pm41751513-75-4-10?v=Novogene
Average 86 stars, based on 1 article reviews
rna sequencing procedures - by Bioz Stars, 2026-07
86/100 stars
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press methods nucleotide sequences guide rna  (Synthego Inc)
86
Synthego Inc press methods nucleotide sequences guide rna
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Press Methods Nucleotide Sequences Guide Rna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pm41741470-228-10-19?v=Synthego+Inc
Average 86 stars, based on 1 article reviews
press methods nucleotide sequences guide rna - by Bioz Stars, 2026-07
86/100 stars
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rna-sequencing procedures  (Riken Genesis Co Ltd)
90
Riken Genesis Co Ltd rna-sequencing procedures
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Rna Sequencing Procedures, supplied by Riken Genesis Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pm40005711-43-6-0?v=Riken+Genesis+Co+Ltd
Average 90 stars, based on 1 article reviews
rna-sequencing procedures - by Bioz Stars, 2026-07
90/100 stars
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rna sequencing procedures  (Azenta)
90
Azenta rna sequencing procedures
CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of <t>bulk</t> <t>RNA-seq</t> data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.
Rna Sequencing Procedures, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pmc11706936-140-1-7?v=Azenta
Average 90 stars, based on 1 article reviews
rna sequencing procedures - by Bioz Stars, 2026-07
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total rna sequencing procedures  (Ebiogen Inc)
90
Ebiogen Inc total rna sequencing procedures
Differentially expressed genes (DEG) detected by <t>RNA-seq.</t> (A) Total <t>RNA-sequencing</t> on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).
Total Rna Sequencing Procedures, supplied by Ebiogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pmc11682960-60-1-7?v=Ebiogen+Inc
Average 90 stars, based on 1 article reviews
total rna sequencing procedures - by Bioz Stars, 2026-07
90/100 stars
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small-rna isolation, library preparation, quality control and next-generation sequencing procedures  (Arraystar inc)
90
Arraystar inc small-rna isolation, library preparation, quality control and next-generation sequencing procedures
Differentially expressed genes (DEG) detected by <t>RNA-seq.</t> (A) Total <t>RNA-sequencing</t> on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).
Small Rna Isolation, Library Preparation, Quality Control And Next Generation Sequencing Procedures, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+sequencing+procedures/pm39562687-42-2-13?v=Arraystar+inc
Average 90 stars, based on 1 article reviews
small-rna isolation, library preparation, quality control and next-generation sequencing procedures - by Bioz Stars, 2026-07
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CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

Journal: Frontiers in Immunology

Article Title: CD32b defines distinct dendritic cell lineages generated from the culture of bone marrow with GM-CSF

doi: 10.3389/fimmu.2026.1703978

Figure Lengend Snippet: CD32b distinguishes transcriptionally and functionally distinct GM-DC subsets derived from MDPs and GMPs. (A) Principal component analysis (PCA) of bulk RNA-seq data from GM-DCs sorted on day 7 and day 21. DCs were generated from cultures of whole bone marrow (BM), sorted MDPs, or sorted GMPs with GM-CSF. (B) Heatmap showing the expression of genes upregulated and downregulated in MDP GM-DCs at day 7, identified through Venn diagram analysis and mapped onto BM GM-DCs at both time points. (C) Volcano plot of differentially expressed genes (DEGs) between MDP GM-DCs (day 7) and GMP GM-DCs (day 21). The thresholds for significance (dotted lines) are set at a log 2 (fold change) ≥ 3 or raw p -value ≤ 10 -16 . (D) Flow cytometric validation of selected surface marker candidates identified in the volcano plot (C) . Normalized Mean fluorescence intensity (MFI) of four representative markers is shown for each DC subset. Normalized MFI was calculated by subtracting the isotype control mean fluorescence from the sample mean fluorescence, and then dividing the result by the isotype control mean. Representative histograms are shown below the bar graphs, with the isotype control overlaid in gray. Data are representative of two independent experiments.

Article Snippet: RNA sequencing (RNA-seq) procedure and analysis were performed by Macrogen (Seoul, Korea).

Techniques: Derivative Assay, RNA Sequencing, Generated, Expressing, Biomarker Discovery, Marker, Fluorescence, Control

Differentially expressed genes (DEG) detected by RNA-seq. (A) Total RNA-sequencing on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).

Journal: Frontiers in Immunology

Article Title: Fexuprazan safeguards the esophagus from hydrochloric acid-induced damage by suppressing NLRP1/Caspase-1/GSDMD pyroptotic pathway

doi: 10.3389/fimmu.2024.1410904

Figure Lengend Snippet: Differentially expressed genes (DEG) detected by RNA-seq. (A) Total RNA-sequencing on Het-1A cells pre-treated with 1% DMSO (control) or fexuprazan (test, FXZ, 30 µM) followed by HCl treatment. (B) Top ten functional categories of DEGs. (C) Volcano plot of cluster analysis for DEGs between immune response and inflammatory response of RNA-seq. (D) Heatmap of pyroptosis-related DEGs on Het-1A cells pre-treated with 1% DMSO (DMSO+HCl_1, DMSO+HCl_2) or fexuprazan (FXZ+HCl_1, FXZ +HCl_2).

Article Snippet: Total RNA sequencing procedures were performed by Ebiogen, Inc. (Seoul, Korea).

Techniques: RNA Sequencing, Control, Functional Assay